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課程名稱 |
生物電子顯微鏡技術學
BIOLOGICAL ELECTRON MICROSCOPY |
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開課學期 |
96-2 |
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授課對象 |
生命科學院 生態學與演化生物學研究所 |
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授課教師 |
黃玲瓏 |
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課號 |
EEB5043 |
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課程識別碼 |
B44 U1550 |
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班次 |
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學分 |
3 |
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全/半年 |
半年 |
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必/選修 |
選修 |
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上課時間 |
星期二3,4(10:20~12:10) |
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上課地點 |
生科419 |
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備註 |
1,2,5堂實習,實習須先與授課老師聯絡
總人數上限：12人 |
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Ceiba 課程網頁 |
http://ceiba.ntu.edu.tw/962EM |
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課程簡介影片 |
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核心能力關聯 |
核心能力與課程規劃關聯圖 |
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課程大綱
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為確保您我的權利,請尊重智慧財產權及不得非法影印
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課程概述 |
1) Feb. 19 Introduction Orientation
2) 26 Specimen Preparation for TEM I Sample Fixation & Embedding, Block Trimming
3) Mar. 4 Specimen Preparation for TEM II Supporting Film & Glass Knife Making
4) 11 Ultramicrotomy Ultramicrotomy I
5) 18 Section Staining Ultramicrotomy II
6) 25 Principle of EM Section Staining
7) Apr. 1 Structure and Operation of TEM Structure and Operation of TEM
8) 8 Cytochemistry & Immunomicroscopy Negative Staining, Shadow-Casting Technique & Lab
9) 15 Scanning Electron Microscopy Analytical Electron Microscopy & Light Micrography
10) 22 Cryo-EM Techniques Freeze-Etching Technique
11) 29 Mid. Examination Cytochemistry & Immunomicroscopy
12) May 6 SEM Sample Preparation Darkroom Technique
13) 13 Image Presentation and Analysis Scanning Analytical Electron Microscopy
14) 20 Fluorescent Microscopy Confocal Microscopy & Campus EM Centers
15) 27 Perspectives of Electron Microscopy Academia Sinica EM Centers
16) Jan. 3 Presentation of EM-Lab Data Individual assistance to student projects
17) 10 Final Examination
18) 17 Hand in Report
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課程目標 |
熟悉各種生物電顯相關技術與原理 |
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課程要求 |
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預期每週課後學習時數 |
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Office Hours |
另約時間 |
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指定閱讀 |
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參考書目 |
(B) References：
1) Electron Microscopy, Methods and Protocols in Molecular
Biology. 2nd ed. (2007) Kuo, John. Humana Press.
2) Electron Microscopy 2nd ed. (1999) Bozzola, j. & L.D.
Russell, Jones and Bartlett Publisher.
3) Electron Microscopy in Biology. A Practical Approach
(1991) Harris,J.R.Oxford University Press.
4) Principles and Techniques of Electron Microscopy-
Biological Applications (1989) Hayat, M. A. 3rd ed. CRC
Press.
5) Artifacts in Biological Electron Microscopy (1988)
Crang R. & K.Klomparens. Plenum Press.
6) The Study of Plant Structure: Principales & Selected
Methods( 1981) O'Brien T.P. & M.E. McCully，Termarcarphi
Pty Ltd.
7) 生物電子顯微鏡學(1991) 陳家全, 李家維, 楊瑞森, 國科會 精
儀中心.
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評量方式
(僅供參考) |
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No. |
項目 |
百分比 |
說明 |
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1. |
實驗成果以及期末口頭與書面報告 |
50% |
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2. |
隨堂表現與測驗 |
10% |
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3. |
期中考 |
20% |
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4. |
期末考 |
20% |
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週次 |
日期 |
單元主題 |
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第1週 |
2/19 |
Introduction ; Orientation |
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第2週 |
2/26 |
Specimen Preparation for TEM ; Sample Fixation & Embedding, Block Trimming |
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第3週 |
3/04 |
Ultramicrotomy ; Supporting Film & Glass Knife Making |
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第4週 |
3/11 |
Section Staining ; Ultramicrotomy I |
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第5週 |
3/18 |
Principle of EM ; Ultramicrotomy II |
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第6週 |
3/25 |
Structure and Operation of TEM ; Section Staining |
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第7週 |
4/01 |
Scanning Electron Microscopy ; Structure and Operation of TEM |
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第8週 |
4/08 |
Cryo-ultramicrotomy ; Negative Staining |
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第9週 |
4/15 |
Cytochemistry |
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第10週 |
4/22 |
In situ Hybridization & Isotope Tracer ; Cytochemistry & Immunomicroscopy |
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第11週 |
4/29 |
Mid. Examination
Cytochemistry & Immunomicroscopy |
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第12週 |
5/06 |
SEM Sample Preparation Darkroom Technique |
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第13週 |
5/13 |
Analytical Electron Microscopy |
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第14週 |
5/20 |
Imaging & Campus EM Centers |
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第15週 |
5/27 |
Perspectives of Electron Microscopy
Academia Sinica EM Centers |
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第16週 |
6/03 |
Individual assistance to student projects |
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第17週 |
6/10 |
Presentation of EM-Lab Data
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